![]() Genome-wide identification of polycomb-associated RNAs by RIP-seq. An RNA code for the FOX2 splicing regulator revealed by mapping RNA–protein interactions in stem cells. HITS-CLIP yields genome-wide insights into brain alternative RNA processing. Essential role for endogenous siRNAs during meiosis in mouse oocytes. Essential role for Argonaute2 protein in mouse oogenesis. Kaneda, M., Tang, F., O’Carroll, D., Lao, K. Divergent RNA-binding proteins, DAZL and VASA, induce meiotic progression in human germ cells derived in vitro. RNA-binding proteins in female reproductive pathologies. Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. Diversity of RNA-binding proteins modulating post-transcriptional regulation of protein expression in the maturing mammalian oocyte. An essential role for the RNA-binding protein Smaug during the Drosophila maternal-to-zygotic transition. A brave new world of RNA-binding proteins. The precise mapping of RBP-binding sites in low-input cells opens the door to studying the roles of RBPs in embryonic development and reproductive diseases. Furthermore, the Ago2 and endo-siRNA complexes fine-tune the transcriptome by slicing long terminal repeat retrotransposon-derived chimeric transcripts. Unexpectedly, transcriptomics and proteomics analysis of Ago2 −/− oocytes revealed that Ago2 interacts with endogenous small interfering RNAs (endo-siRNAs) to repress mRNA translation globally. We determined the binding sites and regulatory mechanisms for several RBPs, including Argonaute 2 (Ago2), Mili, Ddx4 and Ptbp1, in mature mouse oocytes. Here, by coupling RBP-mediated reverse transcription termination with linear amplification of complementary DNA ends and sequencing, we present the LACE-seq method for identifying RBP-regulated RNA networks at or near the single-oocyte level. However, current methods are unable to identify the in vivo targets of a RBP in these low-abundance cells. RNA-binding proteins (RBPs) have essential functions during germline and early embryo development.
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